Technology overview

Nanopore sequencing by Oxford Nanopore Technologies (ONT) is quite novel technology which, however, already has proven its usability for various research purposes. This is a single-molecule method based on the detection of electric current during the passage of nucleic acid through a protein pore. In opposite to common short-read sequencing technologies, like llumina, this allows sequencing of long DNA molecules, or full-length transcripts.


For the purpose of nanopore sequencing we have:

  • 10 MinION sequencers
  • 2 Flongle adapters – for low-throughput sequencing
  • Computational server with multiple GPUs
  • Storage server for keeping all the raw data and basecalled sequences
  • qBit, NanoDrop
  • standard laboratory equipment used for the RNA isolation, purification and library preparation