Prices – Genome Engineering Unit

Price list for mouse lines generation for external clients (internal IIMCB users can find the price list here):

Type of mutation	Basic cost of the service	PRICE The difference in price between academic and commercial users is solely due to the different level of fees independent of IIMCB
Type of mutation	Basic cost of the service	Academic and Non-Profit Institutions	Commercial users
Knock-out mouseline (frameshift or deletion of gene fragment)	PLN 20 135	PLN 20 739	PLN 23 357
Point mutation in essential gene	PLN 32 984	PLN 33 974	PLN 38 261
Point mutation in non-essential gene	PLN 20 566	PLN 21 183	PLN 23 857
Insertion of small-size DNA fragment (up to 880 bp)	PLN 27 830	PLN 28 665	PLN 32 283
Insertion of medium-size DNA fragment (up to 1420 bp)	PLN 36 382	PLN 37 473	PLN 42 203
Insertion of very large DNA fragment (up to 2220 bp)	PLN 46 407	PLN 47 779	PLN 53 832
Insertion of larger DNA fragments (>2220 bp)	PLN 52 706	PLN 54 287	PLN 61 139
Other custom projects (>2220 bp)	Upon request	Upon request	Upon request

Mouse lines can be generated in the following genetic backgrounds: C57BL6/6JRj, C57BL6/J/cmdb, C57BL6/6NRj, BALB/cJRj, B6CBAF1/JRj. Other mouse lines are available upon request and may be subject to additional fees.

The price includes the whole process of mouse line generation.
In particular:

Mutation strategy design
Preparation of proposal to the ethical committee

Preparation of sgRNA, Cas9 mRNA and donor DNA (if used)
Injection of sgRNA and Cas9 mRNA into zygotes of desired genetic background, to generate F0 mutated candidate animals.
Embryotransfer to donor mice
Founder screening
Germline breeding of F0 founder animals

Sequence analysis of germline transmitted N1 animals

Clients are provided several 4-5 weeks old N1 generation (heterozygotic) mice. Price does not include taxes and the cost of shipping.

Insertion of mutations into difficult loci (high GC content, repetitive sequences) may be charged with extra fee.
If harmful phenotype is expected in heterozygotic mutants, extra fee will be applied.