Technology overview

Nanopore sequencing by Oxford Nanopore Technologies (ONT) is quite novel technology which, however, already has proven its usability for various research purposes. This is a single-molecule method based on the detection of electric current during the passage of nucleic acid through a protein pore. In opposite to common short-read sequencing technologies, like llumina, this allows sequencing of long DNA molecules, or full-length transcripts.

Equipment

For the purpose of nanopore sequencing we have:

• 10 MinION sequencers
• 2 Flongle adapters – for low-throughput sequencing
• Computational server with multiple GPUs
• Storage server for keeping all the raw data and basecalled sequences
• qBit, NanoDrop
• standard laboratory equipment used for the RNA isolation, purification and library preparation